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The first such method was developed in biophysicist Steve Quake’s lab at Caltech and commercialized by Helicos Biosciences, but became unavailable after the company declared bankruptcy, saddled by legal issues and unable to find a market niche. These days, the canonical technique comes from a company called Pacific Biosciences (PacBio). Scientists often refer to single-molecule techniques as “third-generation” DNA sequencing, though they’re often also lumped into the NGS bucket with Illumina.